Abstract

IN VITRO PROPAGATION OF CAYRATIA PEDATA VAR. GLABRA GAMBLE. WITH SOME GROWTH REGULATORS USING LEAF AND STEM

Dr. K. Kalaichelvi*

ABSTRACT

Cayratia pedata var. glabra is as old as civilization and through history it has been used as a popular folk medicine. Considering it’s medicinal importance, after looking through its increasing demand, there is a global need to develop quality plant material. In the present investigation, an efficient in vitro propagation technology was developed for a rapid and large scale production of Cayratia pedata var. glabra by using stem and leaf from the mature healthy and approximately 5-6 year old parent plant. After proper sterilization all the explants were micropropagated on MS basal medium supplemented with 3% sucrose as carbon source and 0.8% agar-agar as solidifying agent along with different growth regulators. The maximum callusing frequency and more number of shoot formation was observed in lowerconcentration of BAP (0.5 mg/l) in combination with NAA (0.2 mg/l). The maximum frequency of root formation in leaf callus was 85% and 75% in stem callus and both were achieved on MS medium with NAA (1 mg/l) after two weeks. Therefore, the above protocol could be effectively used in rapid micropropagation of elite plant of Cayratia Pedata Var. Glabra.

Keywords: Cayratia Pedata Var. Glabra, in vitro propagation, BAP, NAA.