Abstract

8-DEHYDROXY CHRYSOPHENOL ISOLATED FROM EXTRACT OF RHEUM EMODI ENHANCE GENTAMICIN INDUCED NEPHROTOXICITY IN RATS MODEL

Mohd Aslam*, Rameshwar Dayal, Kalim Javed, Mohd Samim, Deepak Yadav,SM Arif Zaidi, Surener Singh

ABSTRACT

Revand Hindi (Rheum emodi) is considered as a substitute for Revand Chini. In traditional system of medicine, R emodi used as diuretic, liver stimulant, purgative, cathartic, and stomach disease etc. It is useful in kidney disorders and has been reported in different other treatments. The effects of different extracts (petroleum and chloroform) of R. emodi in gentamicin induced kidney damage were observed, Blood urea nitrogen (BUN), serum creatinine were significantly reduced in petroleum extract while chloroform extract increased the blood urea nitrogen (BUN) and serum creatinine and body weight. Chloroform extract was further fractionated with liquid–liquid extraction with sodium bicarbonate (SB), sodium carbonate (SC) and sodium hydroxide (SH) to find out a fraction which is responsible for toxicity increase by R emodi. When chloroform extracts (CH) shaking with aqueous solution of sodium bicarbonate, a brown ppt was obtained (RE). The chloroform layer after liquid- liquid extraction was coded as CHL. The effects of SB, SC, SH, CHL and RE fractions of chloroform extract of R. emodi in gentamicin induced kidney damaged was observed. Among fractions RE enhance gentamicin toxicity. TLC examination of RE showed a single spot. It was crystallized with methanol, its MASS, IR, 1HNMR were recorded and characterized as 8-dehydroxy chrysophenol.

Keywords: Rheum emodi, Gentamicin, nephrotoxicity, Rat model, NMR, MASS.