Abstract

REVIEW: THE IN-VIVO SCREENING METHODS OF ANTICANCER DRUG

Shirish Patil*, Sudhir Patil, Nitin Kokare and Akshata Pujari

ABSTRACT

Cancer is one of the major life threatening diseases in the world. The available anticancer drugs have distinct mechanisms of action which shows varying effects on different types of normal and cancer cells. Screening methods are routinely and extensively used as concerned with cost and time reduction in drug discovery. The traditional anticancer drug screening methods, including animal experiments and cell-based screening assays. The screening and evaluation procedures for the development of anticancer agents indicated that the entire process which is a difficult task. Presently, active compounds are selected by prescreening and screening against transplanted mousetumors and human tumor xenografts as well as by the in vitro systems. Recently, its role has changed to that of a service screen supporting the cancer research community. Target-based and cell-based screenings for new anticancer drugs in the molecular targeting period are methods of identifying more selective anticancer drugs. Here I review the screening, highlighting several outcomes that have contributed to advances in cancer chemotherapy. Finally, we discuss primary and secondary in vivo evaluation in experimental chemotherapy.

Keywords: Cancer, tumor xenografts, chemotherapy.