Abstract

IDENTIFICATION OF MULTI DRUG RESISTANT GENE IN E.COLI

P. Vigneshwaran*, R. Abishek1, N. Saravanan and N. G. Ramesh Babu

ABSTRACT

This study based up on the isolation of multi drug resistant gene from E.coli. The sample is obtained from the urine of goat and that is cultured by standard quantitative method. From 24 hrs old culture the DNA were separated and PCR is done using that DNA sample. The PCR amplified product is subjected to the RFLP (Restriction Fragmentation Length Polymorphism) for the deduction of desired gene (β lactamase). To confirm that multi drug sensitivity test also has been performed.

Keywords: Multi drug resistance, ?-lactamase, PCR, RFLP.