PHYTOCHEMICAL ANALYSIS AND INVESTIGATION OF ANTIHEMOLYTIC AND ANTIOXIDANT PROPERTIES OF MUCUNA PRURIENS SEED EXTRACT
Anushka Mitra*, Keerthana Kamath and Vaibhavi Vivekanand
ABSTRACT
The phytochemical profiling, antihemolytic activity and antioxidant capacity of seed extract of Mucuna pruriens was demonstrated. Extraction was performed using four solvents (Ethanol, Methanol, Hexane and n-Butanol) by Soxhlet extraction and Magnetic Stirrer methods. Extraction yields were determined but the best results were obtained using Ethanol solvent. Antihemolytic assay demonstrated that the seed extract does not cause lysis of RBCs. EC50 values of the M. pruriens seed extract after performing DPPH radical scavenging assay and Iron chelating assay were found to be 71.48 μg/mL and 70.19 μg/mL respectively.
Keywords: Antihemolytic, antioxidant, Iron chelating, Mucuna pruriens, Phtyochemical, Radical scavenging.
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