Abstract

RAPID IDENTIFICATION OF HUMAN PATHOGENIC BACTERIA (PSEUDOMONAS AERUGINOSA, VIBRIO SPS.) AND FUNGUS (ASPERGILLUS NIGER) BY USING MULTIPLEX POLYMERASE CHAIN REACTION

Akanksha Raj Sriwastava* and Preety Kumari

ABSTRACT

In this study, we focused on the isolation of highly pathogenic bacteria and fungus in freshwater bodies collected from Lucknow region. We have targeted mainly five Vibrio species such as Vibrio cholera, Vibrio alginolyticus, Vibrio mimicus, Vibrio parahaemolyticus, Vibrio vulnificus, Pseudomonas aeruginosa and Aspergillus niger that are the common pathogenic., Aspergillus niger caused lung infection such as cystic fibrosis patients, asthma and aspergilla are the culprits behind allergic bronchopulmonary. In our work we selected five antibiotic such as Oxytetracycline, Rifampicin, Cefotaxime, Tetracycline and Flucanozole used in which Cefotaxime showed highest activity against Vibrio sps and Pseudomonas aeruginosa with a zone of inhibition of 29 mm and 28 mm at 0.2 mg/ml concentration and the Oxyteracycline shows the lowest activity against Pseudomonas aeruginosa with a zone of 19 mm at 0.2 mg/ml concentration. In the fungus sps., Fluconazole showed the highest activity against Aspergillus sps. with a zone of inhibition of 29 mm at 0.2 mg/ml. After genomic DNA isolation, Multiplex PCR technique also provides the highest supplement to the traditional methods for more accurate monitoring of human pathogenic bacteria and fungus in freshwater and soil.

Keywords: PCR, P.aeruginosa, amplification,antibiotic susceptibility, vibrio spp., ZOI.