Abstract

FORMULATION AND EVALUATION OF IN SITU GEL FOR THE TREATMENT OF PERIODONTAL DISEASE

Rahana P. V.* and Dr. Sujith S. Nair

ABSTRACT

Periodontitis is a chronic inflammatory disease affecting supporting tissues of teeth. Conventional treatment is the systemic administration of antibiotics which requires repeated dosing and resulted in toxic adverse effects. These consequences can be solved by delivering the drug at the desired target site to show local therapeutic action. In situ gel-forming systems are polymeric system that exists as flowing solution before administration and undergoes phase transition to form a viscoelastic gel in a physiologic environment. Aim of the present work was to formulate and evaluate an in situ gelling drug delivery system of ofloxacin by cold method using the polymers poloxamer 407 and carbopol. The prepared in situ gels were evaluated for visual inspection, surface pH, viscosity, syringeability, gelation temperature, gelling time, in vitro gelling capacity, drug content and in vitro drug release. The formulation F4 was selected as the optimized formulation which has a gelation temperature of 36.33±0.57◦C with drug content of 95.92±0.13% and showed in vitro drug release of 72.59% at the end of 8 hrs. It follows first order release kinetics with Higuchi model drug release mechanism. The selected formulation was evaluated for its antibacterial activity and stability and it showed good antibacterial activity against both gram positive and gram negative microorganisms and was stable at refrigerated temperature over three months.

Keywords: In situ, Periodontitis, poloxamer, carbopol, ofloxacin, in vitro, thermoreversible.