Abstract

INVESTIGATION OF PHYTOCONSTIUENTS AND HPTLC PROFILE OF CHLOROFORM, ETHANOL AND AQUEOUS EXTRACT OF CASSIA AURICULATA

Shivaprasad Huded*, Ashalatha M. and Gurupadayya

ABSTRACT

Preliminary phytochemical investigation of chloroform, ethanolic and aqueous extracts of cassia auriculata were evaluated and HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software were used. Research study showed the presence of terpenoids, bitter, tannins, saponins, glycosides, amino acids and flavonoids. HPTLC finger printing of chloroform, ethanolic and aqueous extracts of Cassia auriculata powder revealed presence of seven components at the wavelength of 254 and 366 nm. Rf 0.65-.7, 0.85 and 0.79 in chloroform and ethanolic extracts confirms the presence of bitter, steroids and terpenoids respectively. The HPTLC profiles of ethanolic and chloroform at Rf value at 0.6-0.8 and 0.1 for the presence of saponins and tannins respectively using anisaldehyde as spraying agent. The aqueous extract of same plant Rf value were found to be at 0.1-0.39 identifies for the presence of amino acids, using ninhydrin as visualizing agent. It can be concluded that HPTLC fingerprint analysis of seed, flower and seed and flower juice powder extract of Cassia auriculata can be used as a analytical tool for the accurate identification of the plant and it is useful as a phytochemical indicator.

Keywords: Cassia auriculata, Phytochemical Screening, HPTLC Fingerprinting.