Abstract

GC-MS ANALYSIS OF BIOACTIVE PHYTOCHEMICALS PRESENT IN METHANOL EXTRACT OF PLEUROTUS OSTREATUS (JACK EX. FR.) P. KUMM: EVIDENCE FOR ITS MEDICINAL DIVERSITY

G. F. Ramos*, J. O. Umejiego, J. J. Rapales, G. A. Awemu, G. I. Tejano and E. Faller

ABSTRACT

The research study was aimed to determine the chemical constituents present in the fungus Pleurotus ostreatus (Jack ex. Fr.) P. Kumm. methanol extract using Shimadzu P 2010 Ultra Gas Chromatography-Mass Spectrometry. GC-MS analysis of the methanol extract of P. ostreatus revealed the existence of thirty (30) different chemical constituents based from the GC-MS chromatogram. The major chemical compounds detected were at peaks 3, 1, 21, 12 and15 with the following retention times of 2.643, 2.057, 6.717, 4.439 and 4.865 in terms of minutes. The identified major compounds were glycerin (23.36%), formamide (10.62%), 2-nonanol (9.92%), cyclobutanone, 2-methyl-4-hydroxy-(9.67%) and 1,1,2-trimethyl-3,8,9-trioxa-bicyclo[4] (5.62%)respectively. The presence of these major components in P. ostreatus and in all of the other 25 minor constituents shown in the chromatogram indicates their medicinal potentials which may enhance health and wellness in humans for the preservation of human life essential for the restoration of human dignity as they can be formulated into useful and quality drugs to alleviate pains and sufferings in men.

Keywords: GC-MS analysis, bioactive compounds, Pleurotus ostreatus, methanol extract