Abstract

INVESTIGATION OF ANTIPROLIFERATIVE ACTIVITY OF GALANTAMINE PEPTIDE DERIVATE GAL – VAL AGAINST 3T3 CELL LINES

Dobrina Doncheva Tsvetkova *, Danka Petrova Obreshkova , Valentina BojanovaPetkova, Peter Yordanov Atanasov, Rizwana Malik, Sadia Siddiq, BogidaraHadjieva, Milen Ventzislavov Dimitrov

ABSTRACT

In current investigation the aim was the assessment of properties of newly synthesized peptide ester of Galantamine: 6-O-N-[N-(3,4- dichlorophenyl)-D,L-Alanyl]-L-Valil-Glycine-Galantamine (GAL – VAL) to inhibit cell growth rate of cultured 3T3 mouse embryonic fibroblast cells, triplicate treated separately with different concentrations (1.875 М  30 М). The applied MTT assay determines the ability of viable cells to convert a soluble yellow tetrazolium salt [3–(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) into purpleblue formazan, which absorbance is measured spectrophotometrically at λ = 570 nm. Cell growth inhibition (%) and the index of cell viability (%) were calculated. Inhibition of 3T3 cell growth obtained after treatment with GAL – VAL at concentration 30 M is 88.32 %. Inhibitory concentration IC50 is 23 M. These experimental results proved that against 3T3 cell line ester possesses antiproliferative effect.

Keywords: Peptide esters, Galantamine, MTT, 3T3.