Abstract

PHARMACOGNOSTICAL AND PHYTOCHEMICAL EVALUATION OF LEAF OF PREMNA INTEGRIFOLIA LINN

Vd. B. R. Patel*, Varsha G. Singh and Shreedevi

ABSTRACT

Premna integrifolia Linn. is a large shrub or small tree belonging to the family Verbenaceae. The present study deals with the pharmacognostical and phytochemical study of leaf including chromatographic evaluation. Premna integrifolia Linn. leaf is obovate or elliptic, acuminate at apex, entire or finely serrate at margin, cuneate or rounded at base and coriaceous, glabrous in texture. Leaf of the plant can be identified microscopically by the presence of hook shaped and sessile glandular trichomes, starch grains, oil globules, diacytic type of stomata and prismatic crystal. Purity test shows loss on drying (4.508% w/w), total ash (2.215% w/w), alcohol soluble extractive (5.362% w/w) and water-soluble extractive values(14.557% w/w), PH 6.5. Preliminary analysis revealed the presence of carbohydrates, steroid, alkaloids, tannin and phenol. HPTLC study of alkaloid showed the presence of five spots in short and four spots in long UV rays. The information generated by this study provides relevant Pharmacognostical and physico-chemical data needed for proper identification and authentication of leaf of Premna integrifolia Linn.

Keywords: Premna Integrifolia, Trichomes, Starch Grains, Oil Globules.