Abstract

EVALUATION OF CYTOTOXIC ACTIVITY OF ETHANOLIC EXTRACT OF KALANCHOE PINNATA LEAVES WITH BRINE SHRIMP LETHALITY ASSAY

Kaushik Mansi Vijay* and V. N. Garge

ABSTRACT

The Kalanchoe is a genus of plants that are using popular and effective for the treatment of cancer. Its leaves contain an organic compound also exists in the secretions of the toad manages to stop proliferation, self-renewal and propagation of cancer cells. Brine shrimp lethality bioassay is a simple, high throughput cytotoxicity test for bioactive chemicals. It is based on the killing ability of test compounds on a simple zoological organism-brine shrimp. It is a bioassay that appears to be capable of detecting a broad spectrum of biological activities of crude plant extracts. Novel cytotoxic and antitumor compounds can be isolated from potential plant sources through the assessment of cytotoxic activity against brine shrimps. The lethality bioassay using brine shrimp is one of the recent screening technique in the process of drug discovery and development. It is possible to detect and then monitor the cytotoxicity of various crude extracts, by using the brine shrimp lethality bio-assay (BSLA) rather than more tedious and expensive in vitro and in vivo antitumor assays. The study was aimed to evaluate the cytotoxic activity of ethanolic extract of the leaves of Kalanchoe pinnata.

Keywords: Kalanchoe pinnata, cytotoxic activity, ethanol extract, brine shrimp.