Abstract

BIOASSAY-GUIDED FRACTIONATION, MECHANISM OF ACTION AND TOXICOLOGICAL EVALUATION OF ANTI-NOCICEPTIVE PRINCIPLES OBTAINED FROM ERYTHROPHLEUM IVORENSE

Wakeel O. K., Elusiyan C. A., Adewole T. A. and Adeyeba A. O.

ABSTRACT

The present study describes the bioactivity guided identification of anti-nociceptive compounds found in stem bark extract of Erythrophleum ivorense. The stem bark of the plant was air-dried, grounded and macerated using absolute methanol which was then filtered. The filtrate was evaporated using rotary evaporator and Crude Methanol Extract (CME) was obtained. Bioactivity-guided fractionations of CME was performed using partitioning, thin layer, and open column chromatography. Partitioned fractions (20 mg/kg), E. ivorense-fractions (EiF-1 to EiF-6) (20 mg/kg), EiF-1 (5, 10, 20 mg/kg), fractions (F1 to F8) (20 mg/kg), and semi-purified fractions (50 mg/kg) were screened for anti-nociceptive and anti-inflammatory activities. Animals in each group were administered with extract or fractions intraperitoneally. The mechanism of action and toxicological evaluation of semi-purified fraction were also studied. Data were analyzed using One-Way Analysis of Variance (ANOVA) and the level of significance of the data were taken at p

Keywords: Erythrophleum ivorense, Anti-nociceptive, chromatography, partition.