Abstract

EVALUATION OF PHYTOCHEMICAL SCREENING AND CYTOTOXIC ACTIVITY OF DIFFERENT EXTRACTS OF MONOCHORIA HASTATA LEAVES

*Rokeya Akter, Md. Khairul Islam, Rafiqul Islam, Istiak Alam, Md. Abdul Wahab, Md. Habibur Rahman

ABSTRACT

Aims: The aim of this study was to find out the phytochemicals, cytotoxicity activity of the different extracts of. Monochoria hastata leaves. Place and Duration of Study: The study was carried out in September 2017 in the Department of Pharmacy, Jagannath University, Dhaka, Bangladesh. Materials & Methods: Phytochemicals screening performed by different types of tests such as Braymer’s test, Salkowski test, Precipitate test, Molisch’s test,etc. Cytotoxicity activity was determined against brine shrimp nauplii by using the brine shrimp lethality bioassay. Results & Discussion: In phytochemical screening, leaves contain different types of compound such as glycosides, alkaloids, flavonoids, tannin etc. In Brine Shrimp lethality bioassay, after 18hrs later the LC50 of n-hexane extract of the M. hastata was 19.05μg/ml, after 2hours later 6.76 μg/ml and after 24hours later 0.99 μg/ml. After 18hours later the LC50 of CHCL3 extract was 22.90μg/ml, after 20hours later 12.58 μg/ml, after 24hours later 1.89 μg/ml. After 18hours later the LC50 of ethyl acetate extract was 3.28μg/ml, after 20hours later 1.43 μg/ml and after 24hours later 0.148 μg/ml respectively. Conclusion: The plant can be further screened against various diseases in order to find out its unexplored efficacy and can be a potential source of chemically interesting and biologically important drug candidates.

Keywords: Monochoria hastata, Cytotoxicity, Brine shrimp nauplii bioassay.