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A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF L-DOPA AND DIOSGENIN FROM MARKETED APHRODISIAC FORMULATION
Sonal More*, Dr. Varsha M. Jadhav and Dr. Vilasrao J. Kadam
ABSTRACT The present work deals with development of HPLC method for simultaneous estimation of L-dopa and Diosgenin in marketed aphrodisiac formulation containing Mucuna pruriens seed and Tribulus terristris fruit powder as the ingredients. Chromatographic separation of the drugs was carried out on a Hemochrom Instil [(C18 (250 X 4.6 mm, 5 μm)] using the mobile phase ACN: 0.1%orthophosphoric acid in water (20:80 v/v), pumped at the flow rate of 0.6ml/min with UV-detection at 216nm. The two markers were satisfactorily resolved with retention time 3.781 and 5.255 for L- dopa and Diosgenin, respectively. The method was validated and shown to be linear for L-dopa and Diosgenin.The correlation coefficients for L-dopa and Diosgenin are 0.9954 and 0.9961 respectively. The developed method was found to be rapid, simple, specific and reliable for the determination of L-dopa and Diosgenin from these marketed aphrodisiac formulation. Keywords: L-dopa, Diosgenin, HPLC, Validation, ICH Guidelines. [Download Article] [Download Certifiate] |