Abstract

EVALUATION OF EFFECT OF ORGANIC SOLVENT ON CLARITHROMYCIN LOADED PLGA NANOPARTICLES

Ashvini H. M.*, Shivanand K. Mutta and Anudeep Balla

ABSTRACT

Clarithromycin loaded Poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles were prepared by an emulsification-solvent evaporation method. PLGA was employed as a GRAS (Generally Regarded As Safe) polymer. Four different solvent system was used to determine the effect of organic phase solvent on the mean particle sizes of obtained PLGA nanoparticles, different organic solvents like acetone, acetonitrile, ethyl acetate and combination of acetone and ethanol were used with poly(vinyl alcohol) as stabilizers. Prepared nanoparticles characterised for physicochemical parameters in terms of particles' size, poly dispersity index (PDI), entrapment efficiency, release profile, thermal behaviour, and morphology. The particle size analysis results revealed that the smallest particle size of 333.5 nm was obtained with acetone as organic solvent, while large PLGA nanoparticles 875 nm was obtained for mixture of acetone and ethanol as organic phase. Thermal analysis demonstrated the entrapment of drug into the PLGA. Change in organic solvent phase also affected percentage encapsulation efficiency, highest encapsulation was with acetone i.e. 80% and in vitro releases profile consist of initial burst release followed by a slower release.

Keywords: Clarithromycin, PLGA nanoparticles, emulsification solvent evaporation, Particle, organic solvents.