Abstract

ISOLATION AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME HERBAL EXTRACT

Tejaswini Sunil Karande*, Nilam Pawar, Tushar Katkar, Monika Karanjkar, Pawar Bhushan, and Swapnali Khade

ABSTRACT

Background: Medicinal plant Garlic (Allium sativum) and onion (Allium cepa) and Honey (Apismelifera) in which one or more substance or active constituent are present. This constituent are responsible for the chemotherapeutic use. The use of plants in treating ailments has been in practice since a long time ago. Aims: To study antibacterial activity of garlic, onion, honey and garlic-onion–honey combination. Methods: The antibacterial effect of garlic, onion, honey and Garlic-onion-Honey combination against gram positive and gram negative and fungi. Isolates including staphylococcus aureus, Escherichia coli, candidaalbicans all of them use to study antimicrobial activity. Result: Antibacterial activity of these compounds checked by Agar well diffusion method against gram positive and gram negative and fungi. Garlic-Onion-Honey combination has more antibacterial activity, than individual extract of garlic, onion and Honey. Honey shows less antibacterial activity than Garlic and Onion. Conclusion: natural garlic, onion and honey shows antimicrobial activity against bacterial pathogen. The combination of garlic, onion, honey shows higher growth reduction.

Keywords: garlic, onion,honey, garlic-onion-honey combination mixture, antimicrobial activity,