Abstract

POTENTIAL OF POLYOL ON AGING BY MODULATION OF LIVER METABOLIC SYNDROME AND BRAIN NEUROPROTECTIVE EFFECT IN EXPERIMENTAL ANIMALS

*Atef E. Abd El-Baky

ABSTRACT

Generation of free radical and oxidative stress are the basis for changes that lead to age associated functional deterioration and neuronal disorders. Age-related changes lead to neurodegeneration and impairment of hepatic glucose metabolism that associated with peripheral insulin resistance. The role of green tea catechins to amilorate this disorder resulting from ageing was the aim of this work. Method: this study was performed on 60 male albino rats. These rats were divided into six groups (ten in each group), normal three groups (young, adult, old) and another three groups were received green tea catechins (50mg/kg/day) orally for 10 weeks. Results: Both expression of IR-A isoform, mRNA of IRS-2 and hepatic glycogen were decreased in old and adult compared with young rats, while catechins showed no differences between them. The PCR indicated the same results. Blood glucose, insulin and insulin resistance increased in old and adult compared with young rats. Catechins was decrease blood glucose in old, adult and young rats but insulin and insulin resistance not affected. Monoamine oxidase (MAO) was elevated leading to decrease of serotonin and dopamine. Serotonin, dopamine, GSH and SOD were alleviated by catechins in old and young rats. Both neuronal NOS (nNOS) and inducible NOS (iNOS) expression were elevated in adult and old rats and were decreased by catechins. Conclusion: Green tea polyphenols had a beneficial effect on the oxidative damage resulted from the aging process. So, green tea had antioxidant effect, anti-aging actions and protection against aging symptoms as Parkinson's and Alzheimer's disease.

Keywords: Insulin, Monoamine oxidase, Aging, Insulin resistance, Serotonin, dopamine.