Abstract

STUDY OF IN-VIVO ANTI-ANGIOGENIC ACTIVITY OF NOVEL PYRAZOLINE DERIVATIVE BY USING ZEBRA FISH MODEL

Mahesh Kharatmol, Priyanka Chavan, Rushali Chavan, Deepali Jagdale*

ABSTRACT

Angiogenesis is a physiological process in embryogenesis, organ development, endometrial vasculature in menstrual cycle and wound healing. Angiogenesis has also been associated with several pathological conditions such as cancer, arthritis, etc. VEGFR is an important growth factor and a positive regulator of angiogenesis with its distinct specificity for vascular endothelial cells. The zebra fish model has been developed for in vivo screening of anticancer agents having VEGFR inhibitory activity. It has the advantage of in vivo imaging, large progeny and rapid development. Thus, zebrafish provides an attractive model to promote novel cancer drug discovery and reduce the hurdles and cost of clinical trials. The ability of zebrafish to faithfully recapitulate a variety of human cancers provides a unique in vivo system for drug identification and validation. The current work is small effort to check the anti VEGFR activity of synthesized drug by using anti-angiogenic activity in zebrafish model. The synthesized drug was found to have anti-VEGFR activity. Thus the synthesized molecule holds importance as a lead to develop further molecules having anti VEGFR activity and may be screened for its anti pancreatic activity in future.

Keywords: VEGFR, anti-angiogenic, zebra fish, pyrazoline derivative.