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SIMULTANEOUS METHOD DEVELOPMENT AND VALIDATION OF S-AMLODIPINE AND NEBIVOLOL IN A PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD
P. Praneetha Praveen, S. Nagunath, P. USha Sree and S. D. Shanmuga Kumar*
ABSTRACT A simple, rapid, selective, sensitive, accurate and precise HPLC method with UV-Detection method has been developed for S- Amlodipine and Nebivolol and validated in its pure form as well as tablet dosage form. Chromatogrpahic separation was achieved by Sunfire column C18 (4.6x250mm) with 5μm thickness with the mobile phase containing a mixture of acetonitrile: water (60:40v/v). The flow rate was 1mL/min and the detection was carried out 220nm. The RT values of the S- Amlodipine and Nebivolol was found to be 5-25μg/mL and 10-50μg/mL respectively with regression co-efficient (r2) =0.9990. The recovery was found to be 98.0%. Keywords: Nebivolol, S-Amlodipine, RP-HPLC, Accuracy, Linearity, Specificity, Precision. [Download Article] [Download Certifiate] |