Abstract

A STUDY ON THE EXTRACTION, ISOLATION AND CHARACTERIZATION OF PIPERINE FROM PIPER NIGRUM

Darshitha R.*, Niranjan M. S., Chaluvaraju K. C., Mamatha M., Ranjeetha M. and Varsha C.

ABSTRACT

The prolific chemical constituent of Black Pepper, Piperine, is known for its ample biological activities. In the present study an attempt has been made to isolate Piperine using different solvents like Methanol, Chloroform and Isopropyl alcohol in order to prepare its semi-synthetic derivatives bearing 1, 3, 4-oxadiazole scaffolds. Methanolic extract had resulted Piperine equivalent to 7% yield. The percent yield of Piperine from isopropyl alcohol and chloroform extracts were found to be 5% and 3% respectively. 10% w/v potassium hydroxide was used to co-precipitate the resins and acids which are likely to be present along with Piperine. The methanolic extract was subjected to purification and isolation of Piperine by column chromatography using toluene:ethylacetate (8:2) as mobile phase. The isolated Piperine was characterized by melting point, TLC, IR, HPTLC and 1H NMR.

Keywords: Piperine, Methanol, column chromatography, yield, HPTLC.