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World Journal of Pharmacy and
Pharmaceutical Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

Impact Factor : 8.025

ICV : 84.65

WJPPS Citation

	All	Since 2019
Citation	5450	3969
h-index	23	20
i10-index	134	84

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WJPPS: NOVEMBER ISSUE PUBLISHED

NOVEMBER 2024 Issue has been successfully launched on 1 NOVEMBER 2024.

Abstract

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF METHYLPREDNISOLONE IN BULK AND PHARMACEUTICAL FORMULATIONS

Vijaya Krishna C. Aradhya, Greeshma S. E.*, A. Satish Kumar Shetty, Anil kumar

ABSTRACT

The aim of present work was to develop an simple, accurate and precise zero order derivative method and Area Under Curve (AUC) method for the estimation of methylprednisolone and validation according to ICH Q2(RI) guideline. The absorbance maxima was determined at 242nm using methanol as a solvent. The area selected for the estimation of methylprednisolone was between 237 to 247nm. The method represented the the correlation coefficient (R2=0.999) at concentration range 5-25mcg/ml. the method were validated for the parameters such as linearity, accuracy, precision, and limit of quantification, limit of detection as per ICH guidelines. The proposed method were thus found suitable for estimation of methylprednisolone in bulk and tablet formulation without any interference of the excipients.

Keywords: Methylprednisolone, AUC, Validation.

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