Abstract

IN VITRO ANTI-MICROBIAL AND CYTOTOXIC ACTIVITY ANALYSIS OF CISSUS ADNATA IN DIFFERENT FRACTIONS

Tapu Ghosh, Kazi Nuruddin Al Masud*, Md. Nasiful Islam, Tushar Ahmed Shishir, Zarif Morshed, Rezowana Islam, Md. Rafayat Hossain

ABSTRACT

This study provides a scientific basis for the use of Cissus adnata in traditional medicine. The whole plant was extracted using methanol and different partitionates were obtained using different organic solvents, like, dichloromethane, petroleum ether, and chloroform. All these fractions were subjected to cytotoxic activity and antimicrobial activity determination. Among all the partionates dichloromethane fraction provides the highest LC50 value of 6.79 μg / ml. After evaluating the antimicrobial activity of Cissus adnata the methanolic extract show highest effect on different Gram positive bacteria, Gram negative bacteria and different fungal strain as well where Kanamycin was used as standard. Among Gram positive bacterial strains Bacillus subtilis, from Gram negative bacterial strains E. coli, Vibrio mimicus, Vibrio parahemolyticus and from Fungal strains Sacharomyces cerevacae was inhibited greatly.

Keywords: Antimicrobial, Cytotoxicity, Cissus adnata, Disc diffusion assay, Brine shrimp lethality bioassay.