Abstract

PREPARATION AND EVALUATION OF CLARITHROMYCIN LOADED CHITOSAN NANOPARTICLES

Ashvini H. M.*, Shivanand K. Mutta and Anudeep Balla

ABSTRACT

The main objective of this study was to formulate antibacterial (Clarithromycin) loaded chitosan nanoparticles (CNPs). Clarithromycin loaded chitosan nanoparticles was prepared by Ionic gelation method using Sodium TPP as cross linking agent. FTIR and DSC studies ruled out any possibility of interaction between drug and polymers. Prepared nanoparticles characterised for physicochemical parameters. The particle size analysis results revealed that the average particle size of the optimized formulation was 326.8 ± 3.45 nm and the entrapment efficiency was 75.12 % and drug release of about 77% at 72 h. kinetic studies revealed that formulation was more linear towards the zero order plot and Higuchi plot, indicating that the predominant release mechanism was controlled release with diffusion mechanism. The SEM results shown that the prepared particles were had rough surface and spherical in shape, Stability studies indicates nanoparticles stored at temperature of 3-5°C was stable during three months of storage condition. From the present study, it is concluded that chitosan nanoparticles is an effective carrier for the design of controlled drug delivery of poor water soluble drug.

Keywords: Clarithromycin, chitosan nanoparticles, Particle size, in vitro diffusion studies.