Abstract

VALIDATION OF A GAS CHROMATOGRAPHY-HEADSPACE METHOD FOR QUANTITATIVE ESTIMATION OF METHANOL IN WHOLE BLOOD USING ACETONITRILE AS INTERNAL STANDARD

Supriya Krishna*, Dr A. K. Jaiswal and Dr T. Millo

ABSTRACT

The present study evaluates the efficacy of a Headspace Gas- Chromatography with a capillary column as a method for determining the methanol content in Whole Blood. This procedure allows the use of GC-HS for quantitative and qualitative estimation of methanol. The extensive review literature in this field yielded methods with extensive instrumentation and method preparation. Aim of the study was to determine a methodology using Gas Chromatography- Headspace that was suitable for different biological matrix; minimum sample preparation; optimized internal standard and small sample volume. Analysis was performed on whole blood using acetonitrile as internal standard. Peaks were suitably resolved for methanol (1.342min); acetonitrile (2.148min) (IS) within 8.889 min. Linearity was achieved across a concentration of 7.92 mg to 237.6 mg/100 ml with a correlation coefficient of 0.999 for whole blood and standard in DDW. LOQ and LOD were within the range of 2.37-6.33mg% Reproducibility of samples and standards resulted in precision and accuracy of 0.27-6.51%.

Keywords: Methanol, Poisoning; Alcohol, Gas Chromatography, GLC etc.