Abstract

EFFECT OF CIPROFLOXACIN, VITAMIN E AND THEIR COMBINATION ON DNA FRAGMENTATION AND BONE MARROW CYTOGENICITY

El-Sayed M. G. A.*, A. A. A. El-Komy, Hanan M. Sobhy and Shimaa G. Saied

ABSTRACT

The antibacterial activities of the fluorinated 4-quinolone, ciprofloxacin has been ascribed to a marked inhibition of bacterial DNA gyrase. However, evidence is accumulating that ciprofloxacin may cause liver DNA damage. Vitamin E is a free radical scavenger and antioxidant. This study aimed to investigate the influence of ciprofloxacin in recommended dose on liver and spleen deoxy-ribonucleic acid as well as on micronuclei formation in bone marrow erythrocytes and the effects of vitamin E on these changes.Sixty adult male Sprague Dawley rats were divided into four equal groups: control, ciprofloxacin treated, vitamin E treated and ciprofloxacin and vitamin E treated. DNA fragmentation was evidenced in liver and spleen cells of ciprofloxacin treated rats.Micronuclei formation significantly increased in ciprofloxacin treated rats compared to control group. Vitamin E administration reduced liver DNA fragmentation caused by ciprofloxacin in rats. It is suggested that.

Keywords: Ciprofloxacin, Vitamin E, DNA, Cytogenicity.