Abstract

ISOLATION AND IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS AND STUDY OF MULTIDRUG RESISTANCE (MDR-TB)

Dr. Mothanna A. S. Almahdawi, Dr. Ahmmed A. Mankh* and Mohammed Mustfa Kdban

ABSTRACT

The current study aimed to monitor and control the multidrug resistance of Mycobacterium tuberculosis bacteria isolates and to detect its prevalence. The study was conducted at the Institute of Chest & Respiratory Diseases / Baghdad. Different clinical samples were collected from 500 patients to detect the presence of Mycobacterium tuberculosis bacteria by using conventional methods. The direct microscopic examination of Zeihl-Neelsen stained smears showed that 64 (12.8%) out of 500 of the samples were positive for the bacteria. All samples were cultured on Lownstein Jensen (L.J) medium, and 90(18%) of them showed true growth of tuberculosis bacteria. Microscopic examination and culture on Lownstein Jensen medium of sputum sample showed the highest positive percentage (15.9% and 23.1%) respectively, and the results of the biochemical and differential tests showed that all the isolates belonged to M. tuberculosis bacteria. No significant differences were found in the incidence of tuberculosis between males and females (51% and 49%) respectively, while significant differences (P

Keywords: Mycobacterium tuberculosis, isolation, identification, multidrug resista.