Abstract

ASSESSMENT OF ANTIOXIDANT INFLUENCE OF SHORT SERIES PEPTIDES USING HYDROGEN PEROXIDE SCAVENGING ASSAY AND SUPEROXIDE RADICAL SCAVENGING ACTIVITY

Mayuri Mishra* and Kandasamy Nagarajan

ABSTRACT

Peptides 1, 2, 3 and 4 [Arg-lys. (RK), Lys-Hist. (KH), Arg-Arg-Hist. (RRH) and Arg-Arg-Hist. (RRK)] were subjected to in-vitro antioxidant methods for estimation of their potency using Ascorbic acid as the standard drug. Antioxidant methods used were Hydrogen peroxide scavenging (H2O2) assay and Superoxide radical scavenging activity (SOD). The activities of different concentrations of the test drugs were determined and IC50 values of the test compound were subsequently compared with that of standard drug. Among the compound tested in all the methods, the test drug 1[Arg-lys. (RK)], test drug 2 [Lys-Hist. (KH)], test drug 3 [Arg-Arg-Hist. (RRH)] and test drug 4 [Arg-Arg-Hist. (RRK)] showed comparatively better antioxidant capacity with that of the standard drug. Based on the above results, test drug 1[Arg-lys. (RK) and test drug 2 [Lys-Hist. (KH)], could be considered as the best potent drug for various formulations of antioxidant effect suitable for prevention of human diseases.

Keywords: Antioxidant, Peptides, inhibitory concentration, Hydrogen peroxide scavenging assay and Superoxide radical scavenging activity.