Abstract

INVESTIGATION OF ORANGE PEEL ETHANOLIC EXTRACT AS AN ANTICATARACT AGENT

Amol S. Kulkarni, Vikram V. Nimbalkar, Mohammad Zameeruddin, Hajera N. Khan, Sandhya S. Bundel* and Sandip T. Thoke

ABSTRACT

The lens lies behind the iris and the pupil. It works much like a camera lens. It focuses light onto the retina at the back of the eye where an image is recorded. The lens also adjusts the eye’s focus, letting us see things clearly both up close and far away. The lens is made of mostly water and protein. The protein is arranged in a precise way that keeps the lens clear and let’s light pass through it. But as we age, some of the protein and clump together and start to cloud a small area of the lens. This is a cataract. Over time, the cataract may grow larger and cloud more of the lens, making it harder to see a leading cause of blindness and poor vision is a major public health problem worldwide. Diabetes and hyperglycemia have long been recognized as risk factor for cataract. The present study evaluated the in vitro Anticataract activities of orange peel Ethanolic Extract has rich source of flavonoids, show magnificent a Antioxidant activity against glucose-induced cataract genesis using goat lenses. using isolated goat lenses are incubated in artificial aqueous humor and divided into four experimental groups. The orange peel Ethanolic Extract at a dose of 500μg/ml is incubated simultaneously with glucose (55 mM) and glucose (5. 5mM) for a period of 72 h. ascorbic acid (20 μg/ml) is used as the standard drug. At the end of the incubation lense opacity is measured by photographic evaluation. The orange peel Extract shows significant inhibition of cataractogenesis of eye lenses at conc. 500 p.p.m. The present study suggested that the ethanol extract of orange peel possesses Anticataract activity.

Keywords: Cataract, Diabetes, Oxidative Stress, Anticataract Ascorbic Acid, orange peel.