Abstract

INVITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF ANDROGRAPHIS PANICULATA AGAINST UROPATHOGENS

Murugesan Rajeswari*, Sigamani Ajithadevi, Sengodan Kalaivani and Raja Vinodhini

ABSTRACT

The invitro study was undertaken to identify the antibacterial activity and phytochemical analysis of ethanolic extract of Andrographis paniculata against uropathogens. A total of 100 urine samples were collected from the suspected patients and were examined for the identification of bacteria by standard procedures. Of the 100 urine samples, 47 isolates were identified (gram positive and gram negative bacteria) viz., Escherichia coli 21(44.68%), Klebsiella pneumonia 16(34.04%), Pseudomonas aeruginosa 5(10.63%), Proteus mirabilis 3(6.38%) and Staphylococcus aureus 2(4.25%). The antibacterial activity of crude ethanolic extract of Andrographis paniculata was screened by disc diffusion method. The ethanolic extract shown maximum inhibitory activity against Escherichia coli (21 mm), Klebsiella pneumonia (23 mm), Pseudomonas aeruginosa (19 mm) and Staphylococcus aureus (26 mm) but plant extract was fail to show the inhibitory activity against Proteus mirabilis. The results of antibacterial activity of A. paniculata against selective resistant isolates were compared with the results of standard antibiotics. The study result revealed that the antibacterial properties of A. paniculata might be associated with the presence of phytochemical constituents such as, flavonoids, steroids, terpenoids phenols, tannin, oils and resins.

Keywords: Urine, Andrographis paniculata, Phytoconstituents, Antibacterial activity, Disc diffusion method.