Abstract

ELECTROCHEMICAL STUDIES OF MODIFIED 2-HYDROXYPROPYL BETA CYCLODEXTRIN AND MODIFIED CARBON NANOTUBES SENSORS FOR DETERMINATION OF ANTI-DIABETIC PIOGLITAZONE HYDROCHLORIDE

Abdulkareem Abdulraheem, Maha F. El-Tohamy*, Hadi Abbas Alnajjar, Nabeel Alsaffar

ABSTRACT

The present study aimed to suggest a simple, accurate and sensitive electrochemical method for the potentiometric determination of pioglitazone hydrochloride (PLZ). The developed method was conducted by the incorporation of PLZ with sodium tetraphenyl borate (TPB) to fabricate four PLZ-TPB sensors using o-nitrophenyloctyl ether (o-NPOE) as a plasticizer in a polymeric matrix polyvinyl chloride (PVC). Sensor I and sensor II were conventional coated wire membrane type, while sensor II was modified using 2-hydroxypropyl beta cyclodextrin (β-CD) as ionophore. Sensor III and sensor IV were carbon paste type and sensor IV was modified using carbon nanotubes (CNTs). Under optimum condition the proposed sensors displayed Nernstian responses of 53.8±0.8 and 55.5±0.6, 57.1±0.4 and 57.7±0.1mV decade-1 for sensors I, II, III and IV, respectively at 25˚C over drug concentration ranges of 1.0×10-6-1.0×10-3 and 1.0×10-6-1.0×10-2 mol L-1, 1.0×10-7-1.0×10-2 and 1.0×10-8-1.0×10-2 mol L-1 with lower detection limits of 5.0 × 10-7, 4.7 ×10-7 and 5.0 ×10-8 and 4.8 ×10-9 mol L-1 for the four mentioned sensors, respectively. The selectivity of the proposed method towards the investigated drug and some possible interfering species was studied using a separate solution method. The effect of pharmaceutical additives and some related pharmacological action drugs was also tested. The developed method was validated and gave excellent results for the determination of PLZ in its pharmaceutical formulations and biological fluids.

Keywords: Pioglitazone hydrochloride; Carbon paste; carbon nanotubes sensor; 2-hydroxypropyl beta-cyclodextrin; Pharmaceutical dosage forms; Biological fluids.