Abstract

EVALUATION OF CAMPYLOBACTER CONTAMINTION IN POULTRY HOUSES AND POULTRY SLAUGHTERHOUSES IN BAHRI LOCALITY – SUDAN

Elniema A. Mustafa* and Osama Mohamed Suleiman Eshag

ABSTRACT

This study was carried out during the period from April to June 2017 in Bahri locality, Sudan. The overall objective of this study is to evaluate the hygienic biosecurity practices designed to control campylobacter contamination in broiler primary production and processing. Selective media Charcoal Cefoperazone Desoxycholate Agar (CCDA) and Bolton broth media were used to isolate Campylobacter spp. from poultry primary production and primary processing. Sixty samples were collected randomly from two poultry farms (34 fecal and 2 litter samples) and their slaughterhouses (12 carcass after evisceration and 12 after immersion chilling). The percentage of Campylobacter species isolated from total number of samples was 7 (11.7%). The results represented 5 (14.7%) isolates from feacal samples, 2 (16.7%) from pre chill carcasses and no isolates from composite litter and post chill carcasses samples were detected. Also, different species of Enterobacteriaceae were isolated in the medium of Campylobacter. The isolated Enterobacteriaceae were as fallow: 36% Klebsiella spp., 20% Proteus spp., 3.3% E. coli and 28.3% samples were negative to any bacterial growth. With respect to farm samples, fecal samples yielded the highest percentage of positive results for campylobacter spp. (14.7%) when compared to composite litter samples (0%). As to slaughterhouse samples, pre chill carcass samples yielded the highest percentage of positive results for campylobacter spp. (16.7%) when compared to post chill carcass samples (0%). This study indicated that poultry broiler farms had several species of bacterial pathogens during rearing and processing that can grow in Campylobacter medium.

Keywords: Biosecurity, Broiler Farm, Abattoir, Campylobacter.