Abstract

EFFICIENCY OF SOME NUTRIENT SUPPLEMENTS ON BIODEGRADATION OF SPENT ENGINE OIL IN POLLUTED SOIL

N. M. Atef*, H. A. Awad, N. A. Elsawaf, N. Z. Haikal and H. S. Saad

ABSTRACT

The aim of this study was to evaluate the effect of some nutrient samples–chicken manure (CM), Nitrogen, phosphorus, potassium compound (NPK), Nitroben (Azotobacter/Azospirillum) (NT) and Azolla (AZ) as amendments for the bioremediation of oil polluted control soil (PCS) during 120 days. The organisms isolated from oil free soil (OFS) and oil samples were species of: Bacillus, Psuedomonas, Klebsiella, Escherechia , Staphylococcus, Micrococcus, Streptococcus, Burkholderia, Nitrosococcus, Aspergillus, Penicillium, Fusarium, Rhizopus and Mucor. The results of soil analysis showed that CM, NPK, NT and AZ considerably increased the phosphorus andpH of the soil to slightly alkaline condition which favors the biodegradation of the spent engine oil polluted soil. It was also concluded that nitrogen is a necessary nutrient for bacterial biodegradation activities. The highest percentage loss of spent oil was 79.07 by NPK followed by CM, NT and AZ with percentages 77.13, 73.37 and 70.43 respectively. These findings demonstrate the potential of chicken manure, Nitroben, Azolla as well as NPK to considerably increase the biodegradation of the spent lubricating oil polluted soil. In conclusion, the present work suggests the use of chicken manure as potential alternative agents to rationalize spent engine polluted soil as remediation alternative to the expensive chemical and physical methods.

Keywords: Biodegradation; spent engine oil; Chicken manure; NPK; Azotobacter Azospirillum; Azolla,