Abstract

NON-RECOMBINANT MUTAGENESIS OF MANGROVE ISOLATE BACILLUS THURINGIENSIS FOR HYPER PRODUCTION OF ANTICANCER ENZYME L-ASPARAGINASE

Erumalla Venkatanagaraju*, Shiny Bhattacharya and Silpa S.

ABSTRACT

During the past few decades cancer has become one of the most important cause of death. Enzyme therapy in cancer treatment is effective and has been pursued for long time. The significance of microbial anti-cancer enzymes has been proven to be effective and economical agents for cancer treatment. Microorganisms usually have regulatory mechanisms that control the concentration of metabolites synthesized. Therefore, microorganisms cannot synthesize excess of metabolites over limiting cell requirements. The advent of potent mutagenic agents has opened possibilities for enhanced production which may be of interest for specific therapeutic applications. The present investigation has been undertaken to explore the possible hyper production of anti-cancer enzyme L-Asparaginase by subjecting indigenous mangrove isolate Bacillus thuringiensis to various mutagenic agents.

Keywords: L-Asparaginase, Bacillus thuringiensis, Ultraviolet (UV) irradiation, Ethidium bromide (EtBr), Ethyl Methane Sulfonate (EMS).