Abstract

IMPACT OF GINGER DRY POWDER AND ITS METHANOLIC EXTRACT ON NORMAL AND POTASSIUM DICHROMATE RENAL INJURED RATS

Sahar M. Mahmoud* and Faten F. Mohamed

ABSTRACT

The present study evaluates the effect of ginger dry powder (ZDP) and its methanolic extract (ZME) on normal and potassium dichromate (K2Cr2O7)-nephrotoxic rats. Chromium, Cr,(VI) the most toxic form, induces nephrotoxicity associated with oxidative stress in humans and animals. Rats were divided into seven group: (I) served as control, (II), received a single ip injection of K2Cr2O7 (15mg\Kg bw), (III) were administered Zingiber dry powder (ZDP) (200mg\Kg bw), (IV) were treated with ZDP+K2Cr2O7, (V) orally administered TW 20 (10%), (VII) were administered Zingiber methanolic extract (ZME) (200mg\Kg bw) dissolved in tween 20 (10%), (VI) were treated with ZME+K2Cr2O7. ZDP and ZME affected kidney functions, blood urea nitrogen (BUN) and serum creatinine (SCr) levels increased after ZDP administration, while treatment with ZME showed a similar changes. ZDP and ZME to K2Cr2O7-injured rats did not improve BUN or SCr. Changes in kidney oxidant/antioxidant status (malonaldehyde (MDA), glutathion (GSH), catalase (CAT) and superoxide dismutase (SOD) were recorded. Tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) contents were determined as pro-inflammatory cytokines. Histopathology and immunohistochemical studies on proliferator cell nuclear antigen (PCNA), also, ZDP increased degeneration of cells, and deteriorated renal condition in ZDP+K2Cr2O7 treated rats, while ZME decreased degeneration and increased cells generation.

Keywords: Ginger, Potassium Dichromate, Nephrotoxicity, Oxidative Stress, PCNA.