Abstract

6(3,5-SUBSTITUTED-2-BROMO PHENYL) 1,2,4-TRIAZINE DERIVATIVES AS ANTIMICROBIAL AND ANTICANCER AGENTS

Rajeev Kumar*, Ram Kumar Roy and Anita Singh

ABSTRACT

6-(2-Amino-3,5-substituted phenyl)-1,2,4-triazines were prepared by refluxing semicarbazones or thiosemicarbazones in presence of basic medium. 6(3,5-Substituted-2-bromophenyl)1,2,4-triazine derivatives were prepared from 6-(2-amino-3,5-substituted phenyl)-1,2,4-triazine derivatives by using sodium nitrite, hydrobromic acid and Cu2Br2. The structures of compounds were confirmed by FT-IR and H1-NMR spectral data. Compounds were screened for antimicrobial activity against four bacterial strains (Escherichia coli, Bacillus pumilus, Klebsiella pneumonia and Staphylococcus aureus) and two fungal strains (Aspergillus niger and Candida albicans) by using agar well diffusion test. Some compounds showed good antimicrobial activity. The in vitro anticancer activity of compounds was also performed by NCI 60 Cell screen at a single high dose (10–5 M) on various panel/cell lines. In this series, some compounds showed maximum antibacterial and antifungal activities, respectively. One compound was found to be significant active against UO-31, renal cancer cell line with 38.62 % growth inhibition.

Keywords: Antimicrobial activity, anticancer activity, percentage growth inhibition, 1,2,4-triazine derivatives.