Abstract

INVITRO ANALYSIS OF ANTIANNELID ACTIVITY OF MUCUNA PRURIENS AND ALLIUM SATIVUM (GARLIC BULB) AGAINST THE PHERETIMA POSTHUMA (EARTH WORM)

Divya B. J., Pallavi C., Venkataswamy M., Suman B., Eswari B. and Thyagaraju K.*

ABSTRACT

In the present study, an attempt has been made with Hexane, Ethyl acetate, Methanol and water extracts of Mucuna pruriens fresh leaves and Allium sativum bulb cloves to determine the antiannelid activity of traditional medicines. In vitro antiannelid activity of extracts of both plants were determined by their action on Pheretima posthuma (earth worm) at different concentrations. Both the plants exhibited dose and time dependent antiannelid effects or mortality of worms and was recorded with time. Among all water extract showed the best mortality activity in Mucuna pruriens and Allium sativum. The combination of methanol extract of Allium sativum and water extract of Mucuna pruriens showed the potential death rate of earth worms. Thus our data indicates that the methanol and water extracts exhibit potential natural antiparasitic activity. Mucuna pruriens water extract, 100mg/ml, showed 54.31 minutes to cause death and 16.51 minutes to cause paralysis to earth worm. Allium sativum water extract, 100mg/ml, showed 29.46 minutes to cause death and 14.58 minutes to cause paralysis to earth worm by methanol extract. However combination of both plant extracts of (Mucuna pruriens water and Allium sativum water) and (Mucuna pruriens water and Allium sativum methanol) showed paralysis effect of 8 to 11 minutes and death rate time of 24 to 29 minutes. Hence the combination effects of the two plant extracts had decreased paralytic time and death rate time in earth worms. Therefore both plants can be used in regulating the growth of earth worms.

Keywords: Antihelminthic, mortality, antiparasitics, combination.