Abstract

PHYTOCHEMICAL SCREENING, HPTLC ANALYSIS AND IN VITRO ANTIOXIDANT POTENTIAL OF ETHANOLIC EXTRACT OF NELUMBO NUCIFERA SEEDS

S. Sivaranjani, V. Saratha* and S. Usharani

ABSTRACT

The main objective of the present work is to investigate the phytochemical screening, HPTLC fingerprint and in vitro antioxidant of ethanolic extract of seeds of Nelumbo nucifera. Various phytochemical constituents are investigated in N.nucifera seeds. HPTLC analysis showed the presence of flavonoids. The experimental conditions as well as general comments on the application of chromatographic fingerprint analysis are discussed. 1-1 Diphenyl-2- picryl-hydroxyl radical scavenging was carried out to evaluate the antioxidant potential of the extract. Free radical scavenging activity of ethanolic extract of seeds of N.nucifera increased in a concentration dependent manner. The ethanolic extracts possess free radical scavenging activity at five different concentrations (50-250μg/ml). These findings suggested that the ethanolic extract of N.nucifera possess in vitro antioxidant activity and acts as an effective free radical scavenger.

Keywords: Nelumbo nucifera, In vitro antioxidant, DPPH and HPTLC fingerprint profile.