Abstract

CHEMOTHERAPEUTIC EFFICIENCY OF SAPONIN EXTRACTED FROM ARTEMISIA PALLENS WALLS WITH REFERENCE TO DALTON’S LYMPHOMA ASCITES TUMOR MODEL.

G. Renuga* and P. Latha Brindha

ABSTRACT

Plants produce a diverse range of bioactive molecules, making a rich source of different types of medicines. Natural products play an important role in drug development programs in the pharmaceutical industries. Extracts of Artemisia pallens was screening analyzed for their therapeutic capacity against cancer cells. Phytochemical screening of the leaf extract of Artemisia pallens showed the presence of alkaloid, phenols, glycosides, saponin, steroids and triterpene. Purified saponin was administrated in the dalton’s lymphoma ascites tumor induced mice model. The present work is carried out in order to evaluate the efficacy of the saponin as formulated medicine for the treatment of cancer. Saponin significantly increased the survival time in the tumor mouse model by about 50% in comparison with tumor controls. Saponin had a role to decrease the volume of ascitic fluid in tumor-bearing mice by 80%, thereby returning body weight to normal. Cytotoxicity might be reduced due to the protection of white blood cell and platelet counts in ascitic fluid from the tumor-bearing mice were brought to near-normal range. Molecular changes revealed the possibility of apoptosis induction by the saponin treatment in tumor-bearing mice led to a significant reduction in the number of malignant cell clumps for the treated group when compared with the control group, reflecting the potential of saponin to have cytotoxic effects in tumor cells, without affecting normal cells. Antitumor properties of sapanin suggested may be effective alternative drug component in the treatment of cancer.

Keywords: Artemisia pallens, Antitumor, Ascite Dalton’s lymphoma, Sapanin.