Abstract

EVALUATION OF THE CYTOTOXIC EFFECT OF GINGER EXTRACT AGAINST PROSTATE CANCER MODEL USING IN VITRO STUDY

Himayoon Rashid and G. Umamaheswari*

ABSTRACT

Zingiber officinale (Ginger) is a potent source of various bioactive phenolics such as paradols, gingerols, shogaols and gingerones. The multiple benefits of ginger in alleviating inflammation, oxidative stress and proliferation promotes its use as a chemopreventive agent. Here we report the cytotoxic effect of bioactive fraction of ginger extract against prostate (DU145) cancer cell models and the underlying mechanism being involved using a in vitro strategy. The defined extract shows cytotoxic effect in dose dependent manner against cancer cell lines. Further, the ginger extract induced G2M cell cycle arrest in these models to the varying extent. Moreover, the bioactive fraction markedly reduced clonogenic capability of these cancer cells in dose escalating fashion. Lastly, the extract induced DNA fragmentation in these cancer model clearly showing that the cytotoxic effect is due to induction of apoptosis. This work supports the potential use of ginger as an chemotherapeutic agent for tackling distinct cancers.

Keywords: Ginger, Prostate cancer, DU145, Apoptosis, Clonogenic assay, DNA fragmentation assay.