Abstract

ISOZYME ANALYSIS OF ENDEMIC AND ENDANGERED TREE SPECIES SHOREA TUMBUGGAIA OF TIRUMALA HILS

*Dr. R. Vinolya Kumari

ABSTRACT

Shorea tumbuggaia is a species of plant in the Dipterocarpaceae family. It is endemic and medicinally very important plant of Seshachalum hills of India. Now it is in the endangered status. The using of isoenzyme electrophoresis method is useful tool especially in assessment of gene frequency of specific genes, determining of genetic similarities and genetic distances between the two objects. Isozyme analysis is good tools to support conservation and management of forest trees genetic resources. To characterize forest tree stands genetic structure. In this reserch with the isozyme analysis we have compared Mother stands and progeny stands gene flow analysis. With the PAGE electrophoresis, by using Peroxidase, Catalage and SOD. I have developed dendrogram in leaf, root and leaf explants of both invitro and invivo plants. Especially interesting seems to be using of isoenzyme analysis in estimation of gene flow in natural and artificial populations of Shorea tumbuggaia, when the genetic values of artificial management stands is taking into account using of isoenzyme analysis was the step to assess the genetic variation for Shorea tumbuggaia. Isozyme analysis is done in the mother plant by Assay of Peroxidase, Catalase and SOD methods.

Keywords: SOD: Superoxide dismutases; H2O2: Hydrogen Peroxide; PAGE: Poly acryl amide gel electrophoresis.