Abstract

HPLC ANALYSIS FOR METHANOLIC EXTRACT OF JUSTICIA ADHATODA UNDER ELEVATED CO2

S. Saravanan* and S. Karthi

ABSTRACT

Justicia Adhatoda is a well-known medicinal plant in Ayurvedic and Unani medicine that was included in the family of Justicia. It has gained a great interest from the pharmaceutical industry; the alkaloids vasicine from its sap has been shown to be an effective treatment for suppressant, cough and bronchodilator. HPLC analytical methods for the screening of the major in dole alkaloids of J. Adhatoda and their iridoid precursors have been developed. A five month study was carried out in J. adhatoda to see the changes in different concentrations of CO2 with respect to vasicine. UV detector and L-6200 Intel pumps were used for the study. The separation was achieved on C 18 column. The data of first month and the fifth month were compared. When compared to other treatment, control was higher in first month (0.1572 mg ml-1) and it decreased at the end of the fifth month (0.1157 mg ml-1). The vasicine was found to be decreased in the other chambers with 600 ppm (0.1351 mg ml-1), 600 ppm+RH (0.1248 mg ml-1) and 900 ppm (0. mg ml-1) in first month. Whereas at the end of fifth month, it was found that vasicine was present only in control chamber.

Keywords: Justicia Adhatoda, HPLC, elevated CO2, vasicine.