Abstract

ULTRA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY- MASS SPECTROMETRY (UHPLC-MS) ADVANCEMENT IN INSTRUMENTATION AND ITS APPLICATIONS - A REVIEW

Deepika Bairagee*, Sunita Patidar, Rajat Vaishnav and Arun K. Gupta

ABSTRACT

Ultra High Performance Liquid Chromatography Mass Spectrometry is a new category of analytical tool for rapid biomedical analysis with the overall interlaced attributes of speed, sensitivity, rapid, good efficacy and resolution. It has played a significant role in evaluation and interpretation of bioavailability, bioequivalence and pharmacokinetic data. This article reviews the detailed instrumentation of UHPLC-MS its principle and applications. In UHPLC-MS interface plays an important role in the hyphenation technique; as Eluent from liquid chromatography is transferred to mass spectrometer with the help of interface. The development and type of interfaces used in this hyphenation technique are thoroughly discussed. The electrospray ionization (ESI) technique extensively used for production of gas phase ions (without fragmentation) of thermally labile large supermolecules. The charged analytes produced by ESI can be fragmented by activating them in the gas-phase and thus tandem mass spectrometry has been developed, which provides very important insights on the structural properties of the molecule. The mass analyzer such as Quadrupole and Orbitrap used in mass spectrometry are also discussed.

Keywords: UHPLC-MS, ESI, Quadrupole, Analyzer and Orbitrap.