Abstract

COMPARATIVE EVALUATION OF ANTIMICROBIAL ACTIVITY OF SUDANESE HONEY SAMPLES COLLECTED FROM DIFFERENT REGIONS

Mohamed Aldaw Alemam*, Abdelkarim Mohamed Abdelkarim and Eithar Karrar

ABSTRACT

Objective: To assess the in vitro Antimicrobial activity of different samples of Sudanese natural honey collected from various regions against pathogenic microorganisms. Methods: Different samples of Sudanese honey were collected and studied in vitro using standard microorganisms. The antimicrobial activity of the samples was tested and compared against each type of the selected microorganisms. The antibacterial activity of these honey samples was determined by the cup plate method. Results: The mean MIC of different samples of honey for control and test microorganism was 6.7- 8.3 and 12.3- 18.6% (v/v) respectively, the highest diameter of inhibition zone 40 mm (E.coli) was from Honey H2(100mg/ml) and the lowest, 14 mm (Bacillus Subtilis) from Honey H2, while the diameter of inhibition zone for C.albicans 21 mm was from Honey H1(100mg/ml) . Honey collected from Blue Nile State (Ad-Damazin).has shown better antimicrobial activity than other sites. Over all the MIC of all microorganisms was between 6.25-50 and 12.5-100% (v/v) respectively. Conclusions: From the results obtained it can be concluded that all the samples tested possess antimicrobial activity, all collected honey samples showed varied antimicrobial activities, and none of the controlled microorganism were resistant to tested honeys, and can be developed into effective antimicrobial agents.

Keywords: Antimicrobial activity, inhibition zone, Sudanese natural honey.