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BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RIBAVIRIN IN RAT PLASMA BY RP-HPLC METHOD
Dr. Sonali Paresh Mahaparale*, Rasika Pramod Karandikar and Kundan B. Bhalerao
ABSTRACT A simple, rapid, selective, sensitive, accurate and precise High Performance Liquid Chromatography (HPLC) with UV detection method has been developed and validated for determination of Ribavirin in rat plasma. C18 (250x4.6mm) column was used with the mobile phase containing a mixture of Acetonitrile: Water (60:40v/v). The flow rate was 1ml/min and drug was monitored at 218 nm. Plasma samples were processed using acetonitrile as precipitating agent to extract drug. The linearity for Tolperisone hydrochloride was found to be 0.5 to 12 μg/ml with regression coefficient (r2) 0.9990. The recovery was found to be 88.09%. Keywords: Ribavirin, Reverse phases HPLC, Accuracy, Precision, Robustness, LOD, LOQ, and Specificity. [Download Article] [Download Certifiate] |