Abstract

A STUDY ON PRELIMINARY PHYTOCHEMICALS, ACUTE TOXICITY AND DIURETIC POTENTIAL OF LEAVES OF MANGIFERA INDICA L.

Pravin Ramdas Adsul*, Prof. Hemant Jibhau Pagar and Dr. Pandurang Madhukarrao Gaikwad

ABSTRACT

The shade dried powder of Mangifera indica L. leaves were subjected to successive extraction using the solvents (ethyl acetate, 70% ethanol and water) in the increasing order of polarity was prepared. Phytochemical analysis of ethanolic extract of the leaves of Mangifera indica L indicates the presence of alkaloids, tannins, saponins, sterols, phenols, flavonoids, polysaccharides, terpenes. The Aqueous and 70%Ethanolic extracts of Mangifera indica L. leaves were evaluated for diuretic activity. From the toxicity studies 200 mg/kg and 400 mg/kg dose was selected for the diuretic activity. The Lipchitz method is employed for the assessment of the Diuretic activity. The urine output is collected after 24 hours. In the present study, Furosemide (20mg/kg) served as positive control given by intraperetoneal route and the extracts were given by oral route. Na+, K+ and cl- ion concentration in urine were determined by using flame photometer. The treatment of 70% ethanolic extract and Aqueous extract of Mangifera indica L. leaves at 400 mg/kg has increased the urine output significantly, urinary concentration of Na+, K+ and Cl- ions were the parameters of the study. The extracts of 70% EEMI and AEMI at 400mg/kg b.w. has shown good diuretic activity, whereas 200mg/kg dose has shown moderate diuretic activity. From the present study it may be concluded that the Aqueous and 70% Ethanolic extracts of Mangifera indica L. leaves has natriuretic and diuretic activities.

Keywords: Mangifera indica L., Furosemide, Na+/K+ ratio, Diuretic index.