Abstract

TLC- MS HYPHENATED TECHNIQUE IN ANALYTICAL CHEMISTRY

Ashwini B. Sambherao*, Bhushan B. Bhairav and Dr. R. B. Saudagar

ABSTRACT

TLC- MS is a versatile technique for separation as well as identification of Pharmaceuticals and Phytopharmaceuticals. Traditionally the separation was carried out by TLC/ HPTLC then the scrapped materials was removed and then identified by Mass spectrometry. Many hyphenated techniques like HPLC-MS, MS-MS etc. are available. HPLC-MS is more preferred method for separation and identification of compounds. But disadvantage of HPLC is that it requires more solvent as compared to TLC. Therefore taking advantage of less solvent requirement in TLC and also to enhance working hyphenation of both TLC and MS is done so as to provide wide scope for separation as well as identification of product within short period of span. The great advantage of the instrument is that exclusively questioned zones are transferred into the MS for identification and that within less than one minute sensitive mass spectrometric information is available. This review includes Introduction of method, interface for TLC-MS, Advantage with example and Applications of method.

Keywords: Hyphenation, Interface technique, Importance of TLC-MS Coupling.