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THE EFFIECIENT METHOD FOR DNA EXTRACTION FROM BLOOD SPOT ON JEANS
Rasha Sadeq Ameen*
ABSTRACT
When a crime or other incident takes place, clothing items are one of the most common types of evidence that present or left behind in the crime scenes, and can become directly involved in the case itself. Blood sample was collected from (5) voluntaries and spotted on three different color of blue jeans at different time (directly, after 2 and 4 weeks), the DNA was extracted by using the most commonly method (Chelex® 100, organic phenol-chlorophorm, Wizard® Genomic DNA Purification Kit (Promega, USA). Results indicated that the organic phenol- chlorophorm method cannot be used for DNA extraction from the dark blue type of the jeans while there is decrease in DNA concentration with increase in time of spot dry in both chelex and kit methods, in the case of the medium blue type of the jeans the organic Keywords: bloodspots, phenol-chlorophorm organic, chelex® 100, DNA extraction, jeans. [Download Article] [Download Certifiate] |