Abstract

ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF DOMINATED ISOLATES FROM AIR BORN HOUSEHOLD CONTAMINATION

Yashaswi Kain*, Dr. Neeraj Tandan, Pooja Arya and Amar Abhishek

ABSTRACT

In this study, we compare indoor microbial distribution pattern in rural vs urban areas of meerut region. A Very intensive bacterial growth was observed in the kitchen sections compare to living room area of both urban and rural area. The predominant bacterial group and moulds isolated from investigated air samples were: Brevundimonas diminuta; Bacillus megaterium; Bacillus Subtilis; Bacillus ceres, Enterococcus, streptobacillus, Klebsiella pneumoniae; Pseudomonas aeruginosa, Citrobacter freundii and Alternaria spp., Mucur spp., Cladosporium spp., Aspergillus Spp. This result indicates that neither proper cleaning was happened nor air ventilation was maintained in both urban and rural areas. Microbial dominance is depends on intrinsic or genetic adaptation. This work was extended to evaluate the antibiotic susceptibility pattern of dominated bacterial strains. Six bacteria (Brevundimonas diminuta; Bacillus megaterium; Bacillus Subtilis; Klebsiella pneumoniae; Pseudomonas aeruginosa; Citrobacter freundii) showed dominance on all other bacterial and fungal sp. Maximum resistant were found for Vanomycin (Va) and minimum resistance were observed for ciprofloxacin (Ci). Citrobacter freundii found sensitive against for Streptomycin, Ciprofloxacin and Pseudomonas aeruginosa, is found sensitive only for Amoxillin. Moreover, strain Bacillus megaterium, Bacillus Subtilis, Klebsiella pneumoniae, showed multiple drug resistant capability because it resist the entire antibiotic used in this study. The most sensitive strain Brevundimonas diminuta susceptible to antibiotic were negative for plasmid. The antibiotic resistance property was found to be lost in Brevundimonas diminuta indicated the above property is plasmid born.

Keywords: Microbial Load, Bacteria, Fungi, Air Born Microbes.