Abstract

IN-VITRO ANTIOXIDANT ACTIVITY AND ANTI-HYPERLIPIDEMIC EFFECT OF ETHANOL EXTRACT OF MANGIFERA INDICA LEAVES AND BARK ON WISTAR ALBINO RATS

*Omodamiro O. D., Achi N. K. and Ajah Obinna

ABSTRACT

The Anti-lipidemic activity of Mangifera indica leave and bark on rat model and in vitro antioxidant activity of the plant bark was studied. The antioxidant activity was determined using nitric oxide, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and anti-lipid peroxidation free radical scavenging activities. The plant extract showed highest inhibition in nitric oxide (93.5) at the highest concentration 200mg/ml used. This is higher compared to lipid peroxidation and DPPH % scavenger of the plant extract which are (81.5) and (62 ) respectively at the same concentration of 200mg/ml. the % inhibition of the plant bark extract showed low inhibition concentration as against 50% (IC50). Serum lipid assay (total cholesterol, high density lipoprotein, low density lipoprotein, triglycerides and very low density lipoprotein) of the rats were measured to assess the anti-lipidemic activity of both the plant leave and bark extract. The result shows non-significant changes in all the parameters between the control groups and the rats administered with the plant leave and bark extract. It can be deduced from the lipid assay that the administration of the ethanol plant extracts did not cause accumulation of lipid in the serum level of the rats. The LDL-c level was also on the normal range. Therefore this plant can be used as an anti-lipidemic agent.

Keywords: Anti-lipidemic, antioxidant, Mangifera indica, lipid peroxidation, DPPH and nitric oxide.